Thioredoxin Reductase Protein (TrxR) (AA 1-321)

Produktnummer ABIN666984

Dieses Recombinant Thioredoxin Reductase-Protein wird in Escherichia coli (E. coli) exprimiert.

Kurzübersicht für Thioredoxin Reductase Protein (TrxR) (AA 1-321) (ABIN666984)

Target: Thioredoxin Reductase (TrxR)

Protein-Typ: Recombinant

Biologische Aktivität: Active

Spezies: E. coli

Quelle: Escherichia coli (E. coli)

Applikation: SDS-PAGE (SDS), Enzyme Activity Assay (EAA)

Reinheit: > 90 %

Produktdetails

Proteineigenschaft: AA 1-321

Sequenz: MGTTKHSKLL ILGSGPAGYT AAVYAARANL QPVLITGMEK GGQLTTTTEV ENWPGDPNDL TGPLLMERMH EHATKFETEI IFDHINKVDL QNRPFRLNGD NGEYTCDALI IATGASARYL GLPSEEAFKG RGVSACATCD GFFYRNQKVA VIGGGNTAVE EALYLSNIAS EVHLIHRRDG FRAEKILIKR LMDKVENGNI ILHTNRTLEE VTGDQMGVTG VRLRDTQNSD NIESLDVAGL FVAIGHSPNT AIFEGQLELE NGYIKVQSGI HGNATQTSIP GVFAAGDVMD HIYRQAITSA GTGCMAALDA ERYLDGLADA K

Produktmerkmale: TRXB, 1-321aa, E.coli, E.coli

Aufreinigung: > 90% by SDS-PAGE

Biological Activity Comment: Specific activity is > 10unit/mg, and was measured in a coupled assay with DTNB and NADPH. The amount of TNB generated by NADPH was measured in absorbance at 412nm.

Anwendungsinformationen

Applikationshinweise: Optimal working dilution should be determined by the investigator.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Konzentration: 1 mg/mL

Buffer: 20 mM Tris-HCl buffer ( pH 8.0) containing 1 mM DTT, 10 % glycerol

Lagerung: 4 °C,-20 °C,-80 °C

Informationen zur Lagerung: Can be stored at +2C to +8C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Antigendetails

Target: Thioredoxin Reductase (TrxR)

Andere Bezeichnung: trxB

Hintergrund: TRXB (Thioredoxin reductase) is a ubiquitous enzyme which is involved in many cellular processes such as cell growth, p53 activity, and protection against oxidation stress. The mammalian Thioredoxin reductase reduces thioredoxins as well as non-disulfide substrates such as selenite, lipoic acids, lipid hydroperoxides, and hydrogen peroxide. Recombinant E. coli TRXB protein was expressed in E. coli and purified by using conventional chromatography techniques.

Molekulargewicht: 34.6 kDa (321aa) confirmed by MALDI-TOF

NCBI Accession: YP_003044110